Print ISSN: 1813-8497

Online ISSN: 2410-8456

Volume 10, Issue 2

Volume 10, Issue 2, Summer and Autumn 2011, Page 1-104


IN VITRO AND IN VIVO STUDY OF GREEN AND BLACK TEA ANTIMICROBIAL ACTIVITY ON METHICILLIN RESISTANT STAPHYLOCOCUS AUREUS

Raied Taha Yassen; Lammya Kadhum Bakkir; Reham Muhammed

Basrah Journal of Veterinary Research, Volume 10, Issue 2, Pages 1-12
DOI: 10.33762/bvetr.2011.55014

The in vitro study was compared the antibacterial activity of green tea and
black tea extracts against methicillin resistant Staphylococcus aureus (MRSA). Green
tea extract was found to have higher antimicrobial activity on MRSA with an
inhibition zone of 20 mm at a concentration of 10 gm/100 ml. For black tea extract,
the inhibition zone was 15 mm at same concentration .The minimum inhibitory
concentrations for the green tea and black tea were 4mg/ml and 8mg/ml respectively.
The in vivo study of the antimicrobial effect of both green and black tea was
investigated, by subcutaneous inoculation of four rabbits with MRSA bacteria. After
that immediately treated with a series dilution of tested material (green and black tea),
the development of swelling and the degree of necrosis were scored. The green tea
showed marked reduction in the size of swelling less than 2mm at the concentration of
10mg/ ml; also there is a marked reduction in the severity of necrosis. For black tea a
marked reduction occur in the size of swelling (6-10mm) at the concentration of
10mg/100ml; also there is a marked reduction in the severity of necrosis at the same
concentration. This study concludes that green and black tea extracts were showed to
have an antibacterial activity against MRSA and a therapeutic effect against skin
infection caused by MRSA in rabbits

A STUDY ON BLOOD PARAMETERS OF BARBUS XANTHOPTERUS , BARBUS SHARPEYI AND THEIR HYBRID

Abed Al. Majeed H. Talal; Jassim H. Saleh. Amer A. .Jabir

Basrah Journal of Veterinary Research, Volume 10, Issue 2, Pages 13-18
DOI: 10.33762/bvetr.2011.55015

The blood parameters of Red Blood Cells for Barbus xanthopterus, Barbus sharpeyi and B. xanthopterus X B sharpeyi (hybrid )were mentionted. The dimentions of Red Blood Cells ( length and width) for the fish B.xanthopterus, B.sharpeyi and their hybrid were as follows: (6 and 4 microns; 5 and 3 microns; 6 and 4 microns). Hb content values for the above mentioned fish, were as follows: 5.18, 5.323 and 4 g /100 ml.
PCV values for the above mentioned fish were as follows: 68.25% , 100% and 42.85714%. RBC numbers for the above fish were also mentioned as follows: 3450000, 3550000 and 3300000cell/1mm3.

EFFCT OF L-ARGININE ON SPERMATOGENESIS OF THE DIABETIC RAT

Eyhab.R.M.AL-Samawy; Ahmed Sami Jarad; Ahmed Shaker Hisab ALBadran

Basrah Journal of Veterinary Research, Volume 10, Issue 2, Pages 19-32
DOI: 10.33762/bvetr.2011.55018

L-Arginine has hypoglycemic and antioxidant effect in Alloxan diabetic animals
and reduce effect of diabetes complication on spermatogenesis. antioxidant have
essential effect on spermatogenesis, L-Arginine has antioxidant and hypoglycemic
effect. Enhanced oxidative stress and changes in antioxidant capacity are considered
to play an important role in the pathogenesis of chronic diabetes mellitus.
Sixteen mature male rats aged 10 weeks ,were randomly divided into four equal
groups as follows 1st diabetic group (DG) that received 150 mg/kg (IP) Alloxan
single dose ;2nd group diabetic group treated with (L-Arginine-Hcl)(DAG) received
150mg/kg (IP) Alloxan as single dose plus L-Arginine-Hcl 200mg/kg(IP)-per day, 3ed
group treated with(L-Arginine-Hcl)(AG) received 200mg/kg-(IP) perday ,and 4th were
control group(CG) non treated .In 60 day the blood samples collected from heart to
make serological parameters (glucose level) and testes removed to observe their
Histopathological.
Serum Glucose concentration showed a significant increase (p<0.05) in diabetic
group (DG) compared with other groups while diabetic treatment group that has
received L-Arginine 200 mg/kg(IP) (DAG) showed a significant (p<0.05) decrease
compared with (DG).

ANTIOXIDANT, ANTIDIABETIC AND LIPID LOWERING EFFECTS OF CINNAMON AND VITAMIN C IN HYPERGLYCEMIC RABBITS

Ahlam A. Al-Rikaby; and Kassim F. Abdul Kareem; Ala Al-Deen H. Jawad

Basrah Journal of Veterinary Research, Volume 10, Issue 2, Pages 33-48
DOI: 10.33762/bvetr.2011.55021

The study was done to evaluate the antioxidant effects of cinnamon and vitamin C
in controlling hyperglycemia and their effect on lipid profile in male rabbits in
comparison with the effects of insulin therapy and control animals.
Twenty four diabetic rabbits by the injection of alloxan 100 mg/kg body weight
in the marginal vein of the ear. These diabetic rabbits were divided randomly into 4
groups Number of animals in each group = 6:
Group 1: Was given 2 I.U/ animal of insulin subcutaneously daily. Group 2: Was
given ground cinnamon orally 300 mg/kg body weight dissolved in 5 ml normal
saline daily. Group 3: Was given vitamin C orally 200 mg/kg body weight dissolved
in 5 ml normal saline daily. Group 4: Received orally 5 ml normal saline (0.9% Nacl)
daily and considered as control group. All animal groups were treated for five weeks.
Blood samples were taken from these groups weekly for biochemical analysis to
estimate: Blood glucose, Lipid profile (include total cholesterol (TC), triglyglyceride
(TG), high density lipoprotein (HDL), low density lipoprotein (LDL) and very low
density lipoprotein(VLDL) and serum malondialdehyde (MDA).
The results showed high glucose and lipid concentration associated with an
increased oxidant stress alloxan induces on diabetic animals.
The statistically analysis showed that a cinnamon and vitamin C significant (P<
0.05) reduction in glucose and lipid profile (TC, TG, HDL, LDL and VLDL) in
concordance with a significant elevation in HDL (P<0.05). The level of MDA was
also significantly reduced (P<0.05) in all period comparison with period before
treated with vit. C and Cinnamon extract.
It may conclude that, cinnamon and vitamin C have antioxidant activities to cause
an important role in reduction of blood glucose level and lipid profile in
hyperglycemic animals.

THE EFFECT OF PREGNANCY ON SOME PHYSIOLOGICAL PARAMETERS OF IRAQI SHE CAMELS IN COMPARESSION WITH MALES AND FEMALES IN ESTERUS PHASE

Hayder A.N. AL-Zamely

Basrah Journal of Veterinary Research, Volume 10, Issue 2, Pages 49-54
DOI: 10.33762/bvetr.2011.55024

This study was carried out to determine the effect of pregnancy on some physiological parameters in Iraqi She camels in comparative with males and females in estrus phase , sixty (60) camel blood samples were taken from AL-Najaf Slaughter house , twenty (20) blood samples for pregnant She camels , twenty (20) blood samples for females in estrus phase and twenty (20) blood samples for males to study the following parameters , Red blood cells count , hemoglobin concentration and packed cells volume and blood indices which are include mean corpuscular volume , mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration The grossly examination of ovary was used to determine the estrus phase for non – pregnant females . The electronic methods by beck man coulter were used to measure the studded parameters , The results revealed a significant decrease ( P  0.05) in blood parameters which are included RBCS count , Hb concentration and PCV% during pregnancy .
Whereas no significant changes (P  0.05) in the blood indices values. There was decreased during dehydration and return to normal during rehydration , Which don’t take place in any group of this study

ISOLATION AND BIOTYPING OF STAPHYLOCOCCUS AUREUS FROM WHITE CHEESE IN BASRAH LOCAL MARKETS

Nawras N. Jaber

Basrah Journal of Veterinary Research, Volume 10, Issue 2, Pages 55-66
DOI: 10.33762/bvetr.2011.55025

Fifty samples of white cheese were collected from 3 different local market of Basra city AL-basra, (15), AL-ashar(20) and AL-jumhurya(15 ) samples respectively, After being examined by culturing on MSA media , the results reveal thate 53.33% , 50% , and 13.33% of Staph aureus were isolate from white chees respectively . Depending on the biotyping , the percentage of biotype A Staphylococcus aureus was 90% and biotype C was 10% . Antibiotic sensitivety test showed that 55% ,55% , 45% , 35% of isolates were more sensitive to Erythromycin Chloramphenicol Tetracycline and Ciprofloxacin respectively

MORPHOLOGICAL STUDY OF OTOLITH IN TWO MUGILID SPECIES LIZA SUBVIRIDUS(VALENCIENNES,1863) & L.ABU (HECKEL,1843)

Azal Naser Bader Al-nusear

Basrah Journal of Veterinary Research, Volume 10, Issue 2, Pages 67-71
DOI: 10.33762/bvetr.2011.55027

In order to study the otolith (sagitta) of Liza abu (total length ranged 88-120 mm, body weight was from 7 to 9 g) and L. subviridus(total length ranged 155-205 mm, body weight was from 10 to15 g) 20 specimens of each of them were collected from local markets, sagitta was removed from head by making cross section .Some differential characteristics were observed between the two species sagitta in shape of anterior and posterior sides , notch was found in the mid of posterior margin in L.abu, while in L. subviridus the notch locates in posterioventral margin, so sagitta can be used to identify the two species

SURVEY OF AEROMONAS HYDROPHILA IN THREE MARINE FISH SPECIES FROM NORTH WEST ARABIAN GULF, IRAQ

Ghazi M. Al-Maleky; Rafid M. Karim; Amjed N. Al-Abresm

Basrah Journal of Veterinary Research, Volume 10, Issue 2, Pages 72-77
DOI: 10.33762/bvetr.2011.55028

This study preformed on 74 samples of marine water fishes (24 of Acanthopagrus lalus, 27 of platycephelus indicus and 23 of Cynoglossus arel). Which collected from north west Arabian Gulf of Basrah. All samples were examined for the presence of Aeromonas hydrophila in muscles. 24 isolates of Aeromonas hydrophila were obtained, (33.3%) from Acanthopagrus lalus, (44.4%) from platycephelus indicus, and (17.39%) from Cynoglossus arel. Then all isolates were examined for their ability to hemolytic activity as a virulence factor, the higher percentage of hemolytic activity isolates was found in Acanthopagrus lalus

EVALUATION OF SOME EXTERNAL AND INTERNAL EGG QUALITY TRAITS OF QUAILS REARED IN BASRAH CITY

MUDHAR ABD SALMAN ABU TABEEKH

Basrah Journal of Veterinary Research, Volume 10, Issue 2, Pages 78-84
DOI: 10.33762/bvetr.2011.55029

This study was designated to evaluate some external and internal characteristics of quail eggs reared in Basrah city. Ten mature female quails were used in the present study. All birds were reared under similar environmental, managerial and hygienic conditions. To assess the egg quality parameters, a total of 100 eggs were randomly collected for 20 days.
The values related to egg weight, shape index, shell weight, shell ratio , shell thickness, egg length, Egg width, Shell index and shell surface area (cm2) are found respectively as 7.041, 79.590, 0.973, 14.193, 0.307, 2.825, 2.245, 13.044 and 8.205.
The internal traits as albumen index, albumen height, albumen weight, albumen ratio, albumen width, Albumen length, albumin average, yolk diameter, yolk height, yolk weight, yolk index, yolk ratio and Yolk : Albumen ratio are found respectively as 12.056, 3.204, 2.5, 35.515, 2.308, 3.058, 2.683, 2.195, 3.983, 2.591, 17.601, 36.746 and 1.120.

EFFECT OF IMMUNOGLOBULIN Y PURIFIED FROM IMMUNIZED HEN EGGS ON THE GROWTH OF STAPHYLOCOCCUS AUREUS

Ali A. Al-Edany

Basrah Journal of Veterinary Research, Volume 10, Issue 2, Pages 85-96
DOI: 10.33762/bvetr.2011.55030

A novel purification method of egg yolk immunoglobulin (IgY) based on
precipitation using agar-PEG was developed. This method was compared with
chloroform extraction and polyethylene glycol (PEG) precipitation methods. The
results showed the protein contents were high with chloroform method followed by
agar-PEG then PEG method. The purity of resultant IgY was homogeneous with agar-
PEG method followed by PEG method then chloroform extraction method. The IgY
purified by agar –PEG method, obtained from hens immunized by formalin-treated S.
aureus, showed a significant reduction in bacterial growth and the growth inhibition
was dependent on specific-IgY concentration

CLINICAL AND HEMATOLOGICAL STUDY ON OVINE ANAPLASMOSIS IN SULAIMANI PROVINCE- IRAQ

Bahzad. H. salih Mustafa

Basrah Journal of Veterinary Research, Volume 10, Issue 2, Pages 97-104
DOI: 10.33762/bvetr.2011.55032

A study was carried out on Ovine Anaplasmosis in the Sulaimani province of
Iraq from September to December2007. Five hundred thin blood smears were
prepared form Peripheral blood of sheep in 10 different localities and stained by
Giemsa. The prevalence of Anaplasma Ovis was 24 (4.8%) and the rate was highest
8.8% was among animals above 3 years. The infected animal showed mild fever,
hyperpnoea and tachycardia. Hematological values of the infected sheep was as
follows: Packed cell volume 22 ± 4.75 % , erythrocyte count 6.42 ± 1.35 ×106/ μl ,
hemoglobin concentration 6.95 ± 1.28 g/dl , mean corpuscular volume 35.5 ± 5.85/ fl
, mean corpuscular Hemoglobin concentration 11..04 ± 2.42/ pg, mean corpuscular
hemoglobin concentration 31.62 ± 6.62 g/dl and WBCs 11.15 ± 2.18× 10³ /μl , serum
protein value 5.875 ± 0.801 mg/dl, serum calcium 8.341± 1.029 mg/dl, conjugate
serum bilirubin 0.85 ± 0.314 mg/dl and serum cholesterol 57.291 ± 6.153 mg/dl.