Print ISSN: 1813-8497

Online ISSN: 2410-8456

Volume 17, Issue 2

Volume 17, Issue 2, Summer and Autumn 2018


TOXICO-PATHOLOGICAL STUDY OF GENTAMICIN BY INTRAMUSCULAR INJECTION IN EXPERIMENTAL RABBITS

Basrah Journal of Veterinary Research, Volume 17, Issue 2, Pages 1-11
DOI: 10.33762/bvetr.2018.160308

     In the current study, 16 rabbits was divided into two groups.  Animals of Group I (untreated control) were maintained without any treatment. Group 2 animals (treated group), the gentamicin were injected in thigh muscle /I.M. at 25 mg/kg body weight twice a day (morning and evening) for two months. During the experimental period anorexia, emaciation, and death some animals were seen. Histopathological examination showed that there was proliferated of cortical renal tubules cells and degeneration with necrosis of some them as well as congestion of sinusoid in liver and proliferation of bile duct. There was also vacuolated and congestion of myocardial tissue.  In conclusion, the administration of gentamycin for a long time could serve as a source of harm to animal organs such as kidney, liver, bile duct, and heart

BOVINE VIRAL DIARRHEA AND PERSISTENTLY INFECTION OF CATTLE AT NINEVEH PROVINCE, IRAQ

Basrah Journal of Veterinary Research, Volume 17, Issue 2, Pages 14-32
DOI: 10.33762/bvetr.2018.160309

  The current study was conducted to determine the prevalence of Bovine viral diarrhea and persistently infected (PI) animals at Nineveh province ,Iraq,  using antigen capture enzyme linked immunosorbent assay (AC-ELISA) test and multiplex reverse transcriptase polymerase chain reaction (RT- PCR technique) as a diagnostic tool and to determine BVDV genotypes, However, investigation of some epidemiological risk factors associated with occurrence of disease was also encountered. The study was started from January to August, 2017. A total of 494 cattle ear notches (112 adult cows, 30 younger calves and 352 young beeflot calves) was sampled and tested. For investigating of the persistently infected animals, The positive cattle were screened for a second time after four weeks from the first sampling, Moreover, epidemiological data collected through interview with the farmer s’ owner. Furthermore, The 5-Untranslated rejoin (5’ UTR gene) of BVDV extracted from ear notch were used to determine the  genotypes of the virus. Results indicated that the overall prevalence of BVD in Nineveh province based on AC-ELISA and multiplex PCR technique was 5.46% and 13.96%,  respectively.Moreover,  the prevalence of PI in cattle was 0.8% for each test. According to Kappa value (0.457), it has been found that, the multiplex PCR is highly efficient for diagnosis of BVDV in cattle than AC-ELISA test. It was indicated that both  BVDV1 and BVDV2 were detected in Nineveh province at (12.95%, 1.01%) respectively. Results were also indicated that the cattle ages, genders, breeds, importation, large herd size, regions and the seasons were significantly associated with higher prevalence of BVD. It has been concluded that BVD is an endemic disease in Nineveh province, However,  at several factors could assist for its spreading. Moreover, two genotypes were detected, BVDV1 and BVDV2.Therefore, control programs as well as early detection of PI animals should advised, and have the priority.

EFFECTS OF HEXANE OF RICINUS (SEED AND LEAVES )AND BLACK PEPPER HEXANE EXTRACTS ON THE SOME BIOLOGICAL CONTROL OF Chrysomya albiceps

Basrah Journal of Veterinary Research, Volume 17, Issue 2, Pages 33-41
DOI: 10.33762/bvetr.2018.160310

           This study was carried out to investigate the effects of  Hexane extracts of Ricinus (Seed & leaves ) and Black Pepper on Chrysomya albiceps larvae . The result showed that the Ricinus leaves Hexane extract had 100% mortality in all stage Larvae at 0.1&0.5mg\ml and 96.666 mortality in larvae at 1.0mg\ml of the same extract (Table 2). Black pepper Hexane extracts Result showed that 1.0mg\ml had mean mortality 86.666 in larvae (Table 3). Ricinus seed Hexane extract showed 83.333 mean mortality rate at 1.0 mg\ml (Table 1 ).Many concentration were prepared (0.1,0.5,1.0 )mg\ml for each plants extracts in our investigation .Chemicals plants compounds were mage red (Alkaloids, Phenols, Tannins, Resins, and  Oils ). The result showed that’s used of natural plants production had better than the chemicals insecticides which it studied in another time before this our investigation.       

DIRECT EFFECT OF RED LASER IRRADIATION ON TESTICULAR AND EPIDIDYMIS TISSUE FUNCTION IN MALE RABBITS

Basrah Journal of Veterinary Research, Volume 17, Issue 2, Pages 42-50
DOI: 10.33762/bvetr.2018.160396

This study was designed to evaluate the effect of red laser irradiation on testicular and epididymis tissue. Twenty rabbits, male mature were used in the present study. The animals were divided into four groups. First group as control and second, third and fourth groups exposed to red laser irradiation 5 min, 15 min, 30 min respectively. The histological sample was taken after necropsy method of all experimental animals for preparation slide processes to examine under a light microscope. The result of the experiment showed histological changes induced by exposure the animals to red laser (650nm-10w) which present as versus changes and these pathological changes are directly fit with period of exposure which appears as degeneration, oedematous, hyperemia and necrosis into testicular and epididymis.         

EVALUATION OF THE ROLE OF CHITOSAN IN THE IMMUNOPATHOLOGY OF OSTEOMYELITIS IN DIABETIC RABBITS

Basrah Journal of Veterinary Research, Volume 17, Issue 2, Pages 51-69
DOI: 10.33762/bvetr.2018.160397

This study was conducted to investigate the beneficial effects of chitosan in the immunopathology of osteomyelitis in diabetic rabbits; therefore, the experimental design was carried out on 40 rabbits. They were  divided into 5 groups each of 8 animals, diabetes mellitus was induced in rabbits , then infected by Staphylococcus aureus and treated as following: First group (G1) was induced diabetes mellitus then immunized by whole sonicated S. aureus antigens (WSSAG) and induced experimentally osteomyelitis. The second group (G2) was induced diabetes mellitus, then immunized by (WSSAG) and induced experimentally osteomyelitis and fed on diet containing chitosan. Third group (G3) was induced diabetes mellitus, and induced experimentally osteomyelitis only. Fourth group (G4) was induction of diabetes mellitus, and induced experimentally osteomyelitis and fed on diet containing chitosan. Fifth group (G5) was induced experimentally osteomyelitis only without diabetes mellitus induction. Then at day 28th - 30th post immunization, skin test was performed to each of the immunized groups, and at day 30th the antibodies titer was measured by passive hemagglutination assay and phagocytic activity, then the animals were sacrificed and the treated bone taken for histopathological examination. In the present study , a significant increase was noted in the value of skin thickness of G2 at 48 and 72 hrs PI.  A significant increase was also noted in the value of antibodies titers of G2. We also showed a marked decrease   in the t1/2 of carbon clearance of G2. The histopathological results of G2 showed normal periosteal surface and compact bone with active osteoblasts lining the trabecular bones 30 days PI. However, other groups showed many histopathological lesions like infiltration of inflammatory cells and proliferation of fibrous connective tissue in G1, G3, and G4.The results also showed necrotic bone, hemorrhage, inflammatory cells infiltration and fibrosis in G5. Taken together, these findings indicate that the chitosan had a beneficial effect in bone healing of diabetic animals after infection in S. aureus.

HEMATOLOGICAL PROFILE OF RATS TREATED WITH QUERCETIN DERIVATIVE AGAINST CARBON TETRACHLORIDE (CCL4 ) TOXICITY

Basrah Journal of Veterinary Research, Volume 17, Issue 2, Pages 70-84
DOI: 10.33762/bvetr.2018.160398

The effect of quercetin derivative (1-(-2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4 H –chromen-4-ylidene) thiourea coded (QTU)  on the hematological profile of Wistar rats induced toxicity with carbon tetrachloride (CCl4) was investigated.
Administration intraperitoneal injection of 12.5 mg /kg and 25mg/kg of quercetin derivative (QTU), after four weeks of being exposed to CCl4, led to a  significant decrease  (p<0.05) in WBC count, with a corresponding increase on PCV and Hb. It was also observed that the functional recovery of these blood indices is concentration dependent. 
Results of this study revealed a depletion of RBC and a decrease in Packed Cell Volume (PCV) and Hemoglobin (Hb), In addition, an elevation in the levels of White Blood Cell (WBC) caused by CCl4 compared to control samples. 

EFFECT OF IRAQI SWEET ALMOND (Prunus amygdalus) ON SOME PHYSIOLOGICAL AND BIOCHEMICAL PARAMETERS IN ROCK DOVE PIGEONS (Columba livia gaddi) TREATED WITH LEAD ACETATE

Basrah Journal of Veterinary Research, Volume 17, Issue 2, Pages 85-98
DOI: 10.33762/bvetr.2018.160399

The current study aims to evaluate the effect of Iraqi sweet almond (Prunus amygdalus) on the toxicity of lead acetate in wild pigeons Rock Doves (Columba livia gaddi) on body weight and certain hematological and biochemical parameters. Forty healthy adults of both sexes Rock Dove pigeons were randomly selected and categorized into four groups, A,B,C, and D. Group A (control group), were fed on a balanced ration, group B, were administered lead acetate 72 mg/kg body weight orally, group C orally administered with an aqueous extract of Iraqi sweet almond (Prunus amygdalus) 300 mg/kg body weight, and group D were orally administered with 72 mg/kg body weight of lead acetate and 300 mg/kg body weight of the  aqueous extract of Iraqi sweet almond (Prunus amygdalus). These materials were administered daily and for the whole study period which extended to 28 days. Results showed that lead acetate alone caused significant decrease (P≤ 0.05) in the body weight, hemoglobin concentration, total erythrocyte count, PCV% and triglycerides, while there were a significant increase (P≤ 0.05) in total leukocyte count, heterophils, monocytes, lymphocytes, glucose, uric acid, and AST and ALT activities. On the other hand, combined treatment with Iraqi sweet almond and lead acetate to members of group (D) caused significant increase (P≤ 0.05) in hemoglobin, total erythrocyte count, PCV% and lymphocytes, and  significant decrease (P≤ 0.05) in cholesterol and uric acid, while no significant differences in triglyceride and AST and ALT activities. The present finding concludes that adding of Iraqi sweet almond has significant effects on certain hematological and biochemical properties of Rock Dove pigeons (Columba livia gaddi) affected by administering lead.

IMPACT OF GINGER POWDER SUPPLEMENTATION IN BROILER DIET ON THE IMMUNE STATUE DEVELOPMENT AND SMALL INTESTINE MORPHOLOGY

Basrah Journal of Veterinary Research, Volume 17, Issue 2, Pages 99-112
DOI: 10.33762/bvetr.2018.160400

A study using 300 one-day-old Ross-308 broilers was conducted to assess the effects of dried ginger root powder (Zingiber officinale) addition at percentages (0.2, 0.4 and 0.6) % in the treatments T1, T2 and T3 respectively on growth performance, immune status and small intestine morphology of broiler chickens at different ages. Compare with the control group (T0) without adding ginger powder.
    The broiler body weight, body weight gain, viability, production index, and economic profit impacted with addition of ginger powder significantly (P≤0.05) increase in T3 at ages (1-21 d) and (36-42). As well as feed conversion ratio (FCR) particularly improved. In addition the morphological of small intestine goblet cells number in (duodenum , jejunum, and ileum), immunological delayed type of hypersensitivity DTH and ELISA titer against Newcastle(ND), Gumboro (IBD) and Infectious bronchitis (IB) diseases of broiler chickens were significantly higher (P≤0.05) at the ages 21, 35 and 42 d. While mortality and H/L ratio were significantly lower in the treatments of ginger supplementation especially in T3 compare with the control group without ginger addition.

RELATIONSHIP BETWEEN WEIGHT AND MORTALITY RATIO OF COMMON CARP CYPRINUSCARPIOCULTIVATED IN FLOATING CAGES DURING ACUTE INCREASING OF TEMPERATURE

Basrah Journal of Veterinary Research, Volume 17, Issue 2, Pages 113-123
DOI: 10.33762/bvetr.2018.160401

Current study was conducted in 13 floating cages located in Shatt Al-Arab River bank at Al-Hartha District. Common carp Cyprinuscarpio of different weights (290-1700 gm) and numbers (400-1047) fish/cage was cultivated in these cages. Observation was also taken from earthen pond at the same location cultivated with common carp, silver carp Hypophthalmichthys molitrix and grass carp Ctenopharyngodonidella. Results showed that water temperature range between 30-35 0C  during May-June, 2015 and a range of 36- 38 0C during July-August, 2015 in floating cages, while in earthen pond there were 1 0C more. Salinity don’t differ too much during experiment and range between 1.5-3 ppt. Results of earthen pond revealed no fish mortality during experiment for three species cultivated in this pond. Results appeared that fish mortality during May-June don’t largely differed and its ranged between 0.709%-1.861% and there weren’t any relationship between fish mortality and fish weight. Different fish mortality during July-August which ranged between 3.88% for average fish weight of 400 g and total fish mass of 412 kg  and 14.75% for average fish weight of 1700 g and total fish mass of 680 kg. Significant correlation (r=0.86) was found between average fish weight and fish mortality.Significant correlation (r=0.72) was also found between total fish weight and fish mortality. From previous results we concluded that there were positive relationship between fish mortality and fish weight during July-August.

HISTOMORPHOLOGICAL POSTNATAL DEVELOPMENTAL STUDY OF THE OVARIES OF THE LOCAL RABBITS (Oryctologus Cuniculus)

Basrah Journal of Veterinary Research, Volume 17, Issue 2, Pages 124-146
DOI: 10.33762/bvetr.2018.160402

This investigation was carried out to point out structural histomorphological changes that take place in the ovaries of female local rabbits at three different postnatal periods of their life that were kitten, immature and mature does. To conduct such project, 24 rabbits were collected (8 for each age) from the local breeders. Rabbits were euthanized, dissected and subsequently ovaries were collected and fixed with 10% neutral buffered formalin then subjected to routine processing such as dehydration, clearing, embedding and block preparation. Finally, sections of 6 µm were prepared and stained with hematoxylin-eosin and Masson’s trichrome stains. Gross findings showed two functional bilateral elongated triangular-shaped ovaries situated in the abdominal cavity suspended by mesovarian ligament with absence of true ovarian bursa. Microscopic findings revealed prominent structural changes in the ovaries of immature does compared to those of kittens and mature does indicated that immature does after the period of weaning face great developmental growth and changes. The data showed the presence of pre-ovulatory follicles in the mature does and no signs of ovulation because these animals are induced ovulators and that does subjected to the study were all virgin and not allowed for mating with the males.

EFFECTS OF TADALAFIL AND/OR SILYMARIN ON GONADAL FUNCTION IN ADULTS MALE ALBINO RATS

Basrah Journal of Veterinary Research, Volume 17, Issue 2, Pages 147-160
DOI: 10.33762/bvetr.2018.160429

         Taldalafil is a long acting phosphodiesterase-5 inhibitor  widely used in the treatment of erectile dysfunction. The abuse of tadalafil, which is a worldwide problem, is associated with many side effects. Sylimarin considered as a herbal drug, its active constituents  are silybin, isosilybin, silydianin and silychristin. A controversy exist with regard to the effects of tadalafil and silymarin on gonadal function in animal species. The present study aimed to investigate the effects of tadalafil and/or sylimarin on gonadal function adult male rates. Twenty four male rats are  used in the study, they divided into 4 groups (n=6) and gavaged daily for 1 month as follows; 1-control(0.5ml distilled water), 2-tadalafil (10mg tadalafil/kg ), 3-silymarin (100 mg silymarin/ kg) and 4-tadalafil + silymarin ( 10 mg tadalafil and 100 silymarin mg/ kg). Tadalafil significantly increases serum testosterone and decreases LH and FSH, it adversely affect the process of spermatogenesis (sperm count and percentage of motile, dead, and abnormal sperms). While, silymarin significantly increases serum testosterone, LH and FSH and improve spermatogenesis. Serum testosterone remains significantly higher in tadalafil+silymarin group compared with control group, while FSH and LH and parameters of spermatogenesis became insignificantly different from those in control group. 
 
 

ANEW METHOD FOR ESTIMATION MEBENDAZOLE IN ITS PHARMACEUTICAL PREPARATIONS AND IN CAMEL URINE

Basrah Journal of Veterinary Research, Volume 17, Issue 2, Pages 161-172
DOI: 10.33762/bvetr.2018.160430

A simple, accurate and rapid spectrophotometric method has been developed for the estimation of mebendazole in pure, pharmaceutical formulations and camel urine. The method is based on the reaction of mebendazole with1-fluoro 2, 4-dinitrobenzene to produce orange-yellow colored complex having maximum absorption at 494 nm. Beeˈs law was obeyed over the concentration range of 20-280μg/ml, with molar absorptivity of 3.32x104 l/mol.cm. The present method is considered to be simple because it does not need heating, hydrolysis, and solvent extraction steps.
The proposed method has been successfully applied for the determination of mebendazole in pure, pharmaceutical formulations and in camel urine.

EFFECT OF POLICOSANOL EXTRACT ADMINISTRATION ON LIPID PROFILE IN HYPERCHOLESTEROLEMIC FEMALE RATS DURING LACTATION

Basrah Journal of Veterinary Research, Volume 17, Issue 2, Pages 173-182
DOI: 10.33762/bvetr.2018.160431

The present study designed to investigate the effects of the extracted policosanol on the lipid profile of hypercholesterolemic lactating female rats (Ratus norvigicus). Forty-eight female rat (10-12) weeks old, and weighting (200-250 gm) were used in the current study. After daily vaginal smear for 3 sequences estrus cycles, they mated with twenty-four healthy adult fertile males weighting (280-350 gm), 24 of these females were induced to be hypercholesterolemic during the pregnancy, and others still healthy. Sugarcane was collected from Mesan province and policosanol was extracted from sugarcane wax. Immediately after birth, the animals were divided into 6  groups: Group 1 (n=8) (Control): Normal rats were given only 0.5 ml/ animal of Dimethyl Sulphoxide (DMSO) daily immediately after birth. Group 2 (n=8) hyperchol. (HC): Hpercholestrolemic rats were given only DMSO 0.5 ml/ animal daily immediately after birth. Group 3(n=8) (HC+SM): Hpercholestrolemic rats were given simvastatin at dose 20mg/kg BW/ day dissolved in DMSO orally by gavages' immediately after birth. Group 4(n=8) (SM): Normal rats were given only simvastatin at dose 20mg/kg BW/ day dissolve xsqqs ed in DMSO orally by gavages' immediately after birth. Group 5 (n= 8) (HC+ST. pol): Hypercholesterolemic rats were given standard policosanol at dose 20mg/kg BW/day orally by gavages' immediately after birth. Finally, group 6(n=8)( HC+EX. pol): Hypercholesterolemic rats were given policosanol extraction at dose 20mg/kg BW/day orally by gavages immediately after birth.  The treatment continued until weaning ( 30 days). All animals were then sacrificed and blood samples were collected for measuring of lipid parameters. Total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and triglyceride (TG) in all groups. Results revealed a significant improvement in TC level was recorded in (HC+SM) group and no difference was observed as compared with the control group. While the level of TC in (SM), (HC+ST.pol) and (HC+EX.pol) treated groups decreased significantly (p ≤ 0.05) compared with the control group, (HC) and (HC+SM) treated groups. No significant differences in HDL-C were observed between (HC+EX.pol) and control group. While the level of HDL-C still significantly lower in all other treated groups compared with the control group. A significant reduction (p ≤ 0.05) in LDL-C level was recorded in all treated groups compared with (HC) group. No significant differences were observed in LDL-C between (SM), (HC+ST. pol) and (HC+EX.pol) compared with the control group. While the LDL-C still significantly higher (p ≤ 0.05) in (HC+SM) compared with the control group. Levels of triglyceride (TG) in (HC+SM),(HC+ST.pol) and (HC+EX.pol) treated groups recorded a significant elevation ( p≤ 0.05)  as compared with the control group. While levels of TG in (SM) treated group was significantly decreased (p≤0.05) as compared with the control group. Conclusion: local sugar cane can be used to extract the policosanol which has an antihyperlipidemic effect on hypercholesterolemic lactating female rats.

EFFECTS OF DRENCHING AQUOUS EXTRACTS OF LICORICE ROOT (Glycyrrizia galabra)AND Oreganum vulgari on HEMATOLOGICAL CHARACTERISTICS OF ADULT MALE RABBITS (Lepus cunicolus).

Basrah Journal of Veterinary Research, Volume 17, Issue 2, Pages 183-191
DOI: 10.33762/bvetr.2018.160432

    This study was done on thirty mature male rabbits of 1500 gm mean body weight, those rabbits were randomly divided into three equal experimental  groups of ten animals each .The first group was regarded as control group and drenched 3 ml normal saline daily for one month. The second group animals were drenched 3ml of licorice root water extract daily for one month also. While the third group animals were drenched 3ml of water extract of oregano daily for one month also. At the end of the drenching period blood samples were drawn from all animals by heart puncture and blood was analyzed for total RBC count; Hb %; pcv%; total leukocytic  count and blood indices of MCH; MCV, and MCHC were calculated in addition to doing blood smears from each animal to estimate percentages of differential leukocytic count. Results revealed that drenching licorice water extract had a significant increase  (p<0.05) on total RBC count;  total WBC count and neutrophils also, significant decrease in MCHC and eosinophil, whereas results of drenching water extract of Oreganum vulgari caused  significant increase on total leukocytic count , MCV and  neutrophil  while RBC had no significant effect so, PCV, Hb, had no effect in the two experimental.

ISOLATION AND IDENTIFICATION OF SALMONELLA SPP. FROM FECES AND RUMEN OF FARM ANIMALS AND STUDY THE ANTIMICROBIAL SENSITIVITY TEST

Basrah Journal of Veterinary Research, Volume 17, Issue 2, Pages 192-203
DOI: 10.33762/bvetr.2018.160433

 
About 50 samples were collected from November 2016 to March 2017 in city of Basra , from healthy farm animals  the samples were taken from feces and rumen of animals  at  different age ( male and female) and study the antibiotics susceptibility for  five different antibiotics.                 
. (six) isolates (12%) of  salmonella spp were identified by using selective media ( xylose medium and macConky agar), The identification of this bacteria was achieved by using morphological and biochemical characterization (Api20 E system). The total isolation was four samples (20%) from healthy goats, (10%) from sheep and (10%) from cows.All isolates are resistant for ampicillines and Bacitracin ,penicillines but of sensitivity to other antibiotics.
 

THE PROTECTIVE EFFECT OF COQ10, DHEA AND THEIR COMBINATION IN REDUCED EMBRYOTOXICITY AND TERATOGENICITY FOR NORFLOXACIN IN PREGNANT FEMALE RATS

Basrah Journal of Veterinary Research, Volume 17, Issue 2, Pages 204-228
DOI: 10.33762/bvetr.2018.160434

This study was designed to evaluate the protective effect of CoQ10 and DHEA and their combination of norfloxacin induced embryotoxicity and teratogenicity in pregnant female rats and their fetuses. Twenty pregnant rats were divided equally into 5 groups, 4 animals each group as following: Control group (G1): 4 normal pregnant female received orally DMSO 0.5ml/animal/day from 5th - 19th day of gestation, first treated group (T1): 4 normal pregnant female received orally 400 mg/kg norfloxacin once daily, second treated group (T2): 4 normal pregnant female received orally 400 mg/kg norfloxacin once daily and after 1hours injected daily with CoQ10 200 mg/kg IP, third treated group (T3): 4 normal pregnant female received orally 400 mg/kg norfloxacin once daily and after 1hours injected daily with DHEA 25 mg/kg IP, and fourth treated group (T4): 4 normal pregnant female received orally 400 mg/kg norfloxacin once daily and after 1hours injected daily with a combination of CoQ10 200 mg/kg and DHEA 25 mg/kg IP for the same period. Norfloxacin was administered by oral gavages as a single dose. All dams were sacrificed at 20th day of gestation and their fetuses were collected and subjected to morphological and skeletal examinations. The obtained results showed that exposure to norfloxacin in pregnant female rats during gestational period from 5th -19th day demonstrated a significant increased (P≤ 0.05) is resorbed and stillbirth fetuses (dead fetuses at birth), and a significant decrease in fetal body weight, fetal body length, fetal tail length, maternal weight gain and placental weight; and also caused skeletal malformation in all cranial bone compared to the control group. It has been concluded that CoQ10 and DHEA prevented and treated morphological and skeletal anomalies in rat fetuses. Therefore, CoQ10 and DHEA are potential therapeutic antioxidant agents against fetotoxicity and teratogenicity induced by oxidative stress generated by norfloxacin intoxication.

SYNERGISTIC EFFECTS OF ETHYLENE DIAMINE TETRA ACETIC ACID AND IMIPENEM AGAINST Pseudomonas aeruginosa WERE ISOLATED FROM CHEESE IN HILLA PROVINCE.

Basrah Journal of Veterinary Research, Volume 17, Issue 2, Pages 229-238
DOI: 10.33762/bvetr.2018.160435

The study was performed to the study synergistic effect of Ethylene diamine tetra acetic acid  (EDTA) and Imipenem against Pseudomonas aeruginosa were  isolated from cheese at Hilla province. Cheese samples 70 were collected randomly from (Retail, supermarkets and dairy shops) in Hilla and were transported to the laboratory.
    The result revealed (45%)   Pseudomonas aeruginous detecte were collected from cheese. These isolates were tested for disk diffusion method for susceptibility of  imipenem. Eight isolates  were  resistant , and  (84.3%) of Pseudomonas aeruginosa strain were sensitive  (84.3%)  to EDTA alone.
    Also, the result showed of Synergistic EDTA- IMP disc diffusion against (8) isolate of Pseudomonas aeruginosa revealed (87.5%) isolates were sensitive. However; the The result showed significant (P < 0.05) of susceptibility to (8) P. aeruginosa isolates to imipenem with and without EDTA. In conclusion, according to the present study can use oxidant agent inhibition of growth bacteria specific P. aeruginosa such as ethylene diamene tetra acidic acid (EDTA) and can used in preserving food specific sold cheese.

PROTECTIVE EFFECT OF MYRTUS COMMUNIS L.ON ARSENIC-INDUCED PATHOLOGICAL CHANGES ON BRAIN TISSUE OF WHITE RATS.

Basrah Journal of Veterinary Research, Volume 17, Issue 2, Pages 239-251
DOI: 10.33762/bvetr.2018.160436

The study was performed on twelve white rats of approximately of the same body weight ( 200-220 gms). It divided equally into four groups. The 1st group was injected with (10mg/kg B.W) arsenic chloride intraperitoneally, 2nd group was injected with (10mg/kg B.W) arsenic chloride intraperitoneally and (3 mg/ml) myrtle extracts. The 3rd group was normal to control, rats treated orally with(3 mg/ml) myrtle extract only .4th group was injected with (0.2ml) distal water as a control group. At the end of the experiment, the animals were sacrificed, and small specimens(2cm³) were taken from brain to histopathology preparation. The microscopic examination of histopathological sections of the brain of the the1st group was showed severe pathological changes characterized by an area of degeneration, vacuolation of microglia &pyknosis of a neuron also there are edema&hemorrhage.On the other hand, the 2ndgroup showed less pathological changes compared by the 1st group which characterized by less gliosis, less vaculation of microglia with few numbers of astrocyte. The neuron showed normal. while, the 3rd and 4th groups  showed normal neurons, microglial, and astrocyte
 

ASSAY OF TETRACYCLINE IN PHARMACEUTICAL PREPARATIONS, SPIKED INDUSTRIAL WASTEWATER AND CHICKEN MEAT SAMPLES USING VISIBLE SPECTROPHOTOMETER TECHNIQUE

Basrah Journal of Veterinary Research, Volume 17, Issue 2, Pages 252-264
DOI: 10.33762/bvetr.2018.160437

A simple, accurate, and rapid visible spectrophotometric method has been developed for the determination of tetracycline hydrochloride in pure, pharmaceutical preparations and environmental water samples. The method is based on the oxidation of tetracycline hydrochloride by sodium hypochlorite in alkaline medium to form a red colored product with an absorption maximum at 400 nm.  Beer’s Law was obeyed in the range of 2-24 µg/ml with molar absorptivity of 1.346×104 L.mol.-1.cm-1.  The relative standard deviation of the method was less than 2% and accuracy (average recovery ) was  100 ± 0.85 %. The optimum conditions for all color development are described and the proposed method has been successfully applied for the determination of tetracycline hydrochloride in pharmaceutical preparations, chicken meat, and wastewater samples.

A STUDY OF SOME CHEMICAL PROPERTIES OF CANNED FISH PRODUCTS IN LOCAL MARKETS IN BASRAH CITY, IRAQ

Basrah Journal of Veterinary Research, Volume 17, Issue 2, Pages 265-276
DOI: 10.33762/bvetr.2018.160438

Concentrations of seven heavy metals (Cadmium, Lead, Zinc, Nickel, Iron, Copper, and Manganese) in five canned fish samples found in the local market Basrah city, Iraq were determined after digestion according to the Association of Official Analytical Chemists methods. Concentrations of metals were measured using the Flame Atomic Absorption Spectrophotometer. The results showed that the range obtained for the elements analyzed in mg/kg (dry weight) were as follows, Cd (0.0027 - 0.0078), Pb (0.0048 - 0.0093), Zn (0.0103 - 0.0205), Ni (0.0122 - 0.0128), Fe (0.0149 - 0.0237), Cu (0.0008 - 0.0034) and Mn (0.0014 - 0.0037). The results indicated that canned fish samples in this study have concentrations well below the permissible FAO/WHO levels for these toxic metals.