Print ISSN: 1813-8497

Online ISSN: 2410-8456

Volume 16, Issue 1

Volume 16, Issue 1, Winter and Spring 2017, Page 1-156


HISTOLOGICAL AND MOLECULAR STUDY OF SPLEEN IN Japanese Quail UNDER THERMAL CONDITION

Zainab A. Al-Ali Majdy Faisal Jalal yaseen

Basrah Journal of Veterinary Research, Volume 16, Issue 1, Pages 1-10
DOI: 10.33762/bvetr.2017.127303

The present study was conducted to determine the histological and Molecular
changes in the spleen of adult male Japanese quail after being exposed to effect of
thermal stress was recorded. This study included two groups, each group consisting
twelve male birds, the control group (A) was exposed to normal temperature for
45days, while the group (B) exposed to temperature (42OC) for 45 days. The
histological and molecular changes were studied during (15, 30 and 45) days of the
experiment, histological changes in spleen were represented by necrosis,
degeneration hemorrhage and shrinkage fibrosis of lymphocytic nodules,
cytoplasmic vaculation, infiltration of lymphocytes, metamorphosis of lymphocyte,
nuclei pyknotic, dilation in the central vein of lymphatic nodules, edema, dilation of
sinusoids and congested blood vessels. Molecular examination showed that the heat
shock protein (hsp70) gen is present in temperature group that is also found in the
control group. This confirms that the hsp70 gen is present in birds at normal and
abnormal conditions.

WANDERING THYROID FOLLICLES WITHIN THE RENAL TISSUE OF CARP FISH "Cyprinus Carpio".

Amer M. Hussin

Basrah Journal of Veterinary Research, Volume 16, Issue 1, Pages 11-19
DOI: 10.33762/bvetr.2017.127386

Twenty healthy adult Carp fish were collected from Dijlah river in Iraq in
Summer and winter times of the year (Ten for each ). The fish were sacrificed and
fixed in 10% neutral buffered formalin solution. Routine histological preparations
were carried out. Hematoxylin - Eosin stain was used. The finding of the current study
showed that clear connective tissue capsule was not observed. Instead, wide loose
connective tissue was surrounded the follicles. The thyroid follicles were distributed
individually or in groups in the kidney and sub pharyngeal region( around the ventral
aorta). The thyroid tissue was represented by thyroid follicles of different sizes and
shapes. In Carp fish, the building up and organization of the follicular cells of thyroid
follicles were simple and poorly organized. There was a close relationship between
the morphological state of thyroid follicles and the season( temperature). The results
of the present study also demonstrated that the spread of thyroid follicles in summer
was more than in winter where the follicles appeared as aggregated at the periphery
of renal tissue and also the results of this study showed that large and small follicles
were combined. The study also registered that, during winter, the large follicles were
mostly formed by the aggregation of smaller ones and that according to the
physiological status of the thyroid tissue. The study concluded firstly that these
follicles had the ability to wandered within the renal tissue. Moreover, the study
concluded that the thyroid follicle was dynamic structure, and their sizes depending
on the amount of colloid present within .

STUDY THE EFFECT OF FLAX LIGNAN EXTRACT OF LINUM USITATISSIMUM AND CONJUGATED ESTROGEN ON PHYSIOLOGICAL PARAMETERS IN FEMALE RATS

Muna H. AL-Saeed; Eman A. AL-Masoudi; Wafaa H. Haran

Basrah Journal of Veterinary Research, Volume 16, Issue 1, Pages 20-49
DOI: 10.33762/bvetr.2017.127387

This study was carried out in the animal house of the College of Veterinary Medicine /Basrah University to evaluate the effect of lignan extract of flax seed on haematological, biochemical parameters and histological examination in female rats, compared with conjugated estrogen drug.
For this purpose, eighteen female rats weighed (180-200g) and aged (4-5 months) was divided into 3 equal groups (6 rats / group) for 14 days.
First group: Drenched 1ml of normal saline (0.9% of NaCl)for 14 days.
Second group: Drenched 0.10mg/kg B.W of conjugated estrogen dissolved in normal saline (0.9% of NaCl) for 14 days.
Third group: Drenched 20mg/kg B.W of lignan extract of flaxseeds dissolved in normal saline (0.9% of NaCl)for 14 days.
Blood samples were collected from heart by cardiac puncture from all experimental animals. These samples are used for the measurement of haematological and biochemical parameter as well as hormonal assy. Thyroid glands, liver, kidneys, ovaries and uterus were removed for histopathological study.
The results revealed that, the female rats treated with lignan extract of flax seeds showed an increase in body weight.
The result also showed that treated female rats with phytoestrogen had no significant effect on blood parameters except a significant effect on lymphocytes. (P≤ 0.05) in serum lipid profile except HDL compared to control.


Moreover the results of biochemical parameters were indicated the affected in female rats treated with lignan extract that the revealed a significant(P≤ 0.05) decrease in serum lipid profile except HDL-Ch of female rats treated with lignan extract compared with the control group. Also the treatment with lignan extract caused significant (P≤ 0.05) increase in LH hormone, estradiol and progesterone compared with .While it has no significant effect on FSH.
The treatment showed some ameliorative effect on histological structure of studied organs.

HORMONAL PROFILE OF IRAQI BITCHES DURING VARIOUS PHYSIOLOGICAL STAGES.

Nazih Wayes Zaid

Basrah Journal of Veterinary Research, Volume 16, Issue 1, Pages 50-66
DOI: 10.33762/bvetr.2017.127388

The study was conducted to investigate the success of inducted estrus and to
track the hormonal levels during the estrus cycle, pregnancy and post-parturition in
dogs. Ten females, aged 2-3 years and weighted 18-22 kg, were kept in cages at the
College of Veterinary Medicine-University of Baghdad. Dogs were mated and tracked
till they become conceive. Then, after one month of parturition, they treated with
Bromocriptine (Parlodel) a dose of (0.05mg/kg/dog) twice daily to induce estrus.
Eight dogs showed estrus and mated again with the same mature dogs. Blood serum
was taken once a week from cephalic vein. Follicular Stimulating Hormone (FSH),
Luteinizing hormone (LH), Estrogen and Prolactin were measured by using canine
Gamma counter kits. In both, normal and induced estrus periods, FSH and LH
hormone levels started to elevate in proestrus and estrus phases then they declined in
pregnancy months and post-parturition. Estrogen and prolactin hormones had
significant differences during the second month in the other non-responsive dogs.
Estrogen levels indicated that the proestrus, estrus and treatment period showed
higher significant differences (P<0.01) than anestrus period.

THE EFFECT OF GALLIC ACID AS PROTECTIVE AGENT ON SOME BIOCHEMICAL PARAMETERS INDUCED HYPERCHOLESTEROLEMIA IN LABORATORY MALE RATS

Muna F. Abass AL-Taei; Mohammad A. AL-Diwan

Basrah Journal of Veterinary Research, Volume 16, Issue 1, Pages 67-79
DOI: 10.33762/bvetr.2017.127437

The role of gallic acid was studied to alleviating some biochemical parameters
alterations in hyper cholesterolemia in rats. For this purpose 24 sexually adult male
rats were divided randomly in to 4 groups (6 rats each). The experiment was
conducted at the animal house of the Veterinary Medicine College–University of
Basrah, the control group, rats were injected intraperitoneally ( I.P.) with 0.9 %
normal saline (N.S)and were supply with the stander ration.
The first treated group were injected intraperitoneally ( I.P.) with 100 mg/ kg
gallic acid dissolve in 0.5 ml distilled water daily and were supply with the stander
ration.
The second treated group were supplied with the stander ration in addition to
1.5% cholesterol of the stander ration .
The third treated group were supplied with standard ration in addition to
1.5% cholesterol, and injected intraperitoneally ( I.P.) after one hour with 100 mg/kg
gallic acid dissolved in 0.5 ml distilled water
The obtained results indicated that the administration gallic acid as protective
agent on high cholesterol diet tend to alleviated and improved lipid profile by a
significant reduction in the serum total cholesterol
(TC),LDL-C and VLDL compared with animals fed cholesterol 1,5% diet, hepatic
enzymes activates of AST &ALT in the protective group almost were at the range of
normal values and the protective urea blood value reach almost as the normal level as
control group.

PREVALENCE OF BRUCELLOSIS IN BUFFALOES OF BASRA GOVERNORATE, BASRA - IRAQ

Mustafa Q. Hasoon; Mohammed A.Y. Al-Amery

Basrah Journal of Veterinary Research, Volume 16, Issue 1, Pages 80-86
DOI: 10.33762/bvetr.2017.127438

The present study was conducted to identify the prevalence of brucellosis
among buffaloes in Basra governorate, via examination of serum samples from 250
she buffaloes reared in different Basra reigns. Sera were examined firstly by rose
Bengal test (RBT) followed by indirect enzyme linked immunsorbent assay (Elisa).
The result of RBT indicated that from 250 buffaloes serum samples there were
27(10.8%) positive against Brucella abortus antigen.
Elisa test was performed on 88 sera samples that included a 27 RBT positive
sera and other 61 negative sera, and the result revealed that 21( 23.8%) seropositive
sera for Brucella abortus. According to the regions of Basra Governorate the
percentage rate of brucellosis were indicated in: Al Hartha 6(5,28%) then Al Qurna
5(4,4%), Al Dear 4(3.5), Al Zubair 3(2.6%), Al Medaina 2(1.76%) and Al Tanooma
1(0.88%). More over, infection in older animals found more significant ( P < 0.05)
than in youngness, beside that infection rate were high in pregnant buffaloes in
compared with non pregnant animals.
Conclusion: the brucellosis of buffaloes in Basra governorate were caused by B.
abortus and were more prominent in pregnant animals, therefore animals screening
of suspected animals was advised,

PHYSIOLOGICAL AND BIOCHEMICAL CHANGES FOR 5- FLUOROURACIL DRUG (ANTI-CANCER) IN CANCER PATIENTS

Baida R. Ali

Basrah Journal of Veterinary Research, Volume 16, Issue 1, Pages 87-99
DOI: 10.33762/bvetr.2017.127439

The study was conducted to determine the effect of the 5-Fluorouracil (5-FU) drug is a
medication used to treat cancer or the knowledge of 5-FU drug(anticancer) effect might help to
attribute these side on physiological and biochemical parameters in human by injection into a vein it is
used for colon cancer, esophageal cancer, stomach cancer, pancreatic cancer, breast cancer and cervical
cancer. 80 blood samples were obtained adult human divided into two groups for each group (n = 40),
the first group (control) and the second group treatment with drug 5-fluorouracil.
There are a significant decrease (P <0.05) in the blood parameters included parameters Red
blood cells count, the total number of white blood cells, platelets , hemoglobin and packed cell volume
in men treatment with 5-fluorouracildrugcompared with control group. Causing the 5-FU drug
significant decrease(P<0.05) in Triglycerides, cholesterol levels and total protein in the treatment group
compared with control group. Also, the result showed a significant increase in Creatinine level of
second group in compared with control group, while the results showed non- significant in urea level in
human treatment when compared with control group.
The results showed a significant decrease (P <0.05) in the level of liver enzymes, (ALT AST and
ALP) in the treatment group with 5-FU when compared with the control group.

PATHOPHYSIOLOGICAL EVALUATION OF THE PROTECTIVE ROLE OF ALPHA LIPOIC ACID ON DIABETES MELLITUS NEPHROPATHY IN MALE RABBITS

Jihad Abdulameer Ahmed

Basrah Journal of Veterinary Research, Volume 16, Issue 1, Pages 100-119
DOI: 10.33762/bvetr.2017.127440

In order to estimate the ameliorating effects of alpha lipoic acid (ALA) on the
nephropathy of diabetes mellitus (DM) associated with oxidative stress, this study
performed in 18 adults male rabbits were divided into 3 equal groups, the group-I was
regard as control while group-II Induced DM only by intravenously single dose (150
mg/kg b.w.) of alloxan-monohydrate; group-III was induced DM by intravenously
single dose of (150mg/kg b.w.) of alloxan-monohydrate and then treated by (10mg/kg
b.w.) daily of ALA intraperitoneally for 4 weeks. The biochemical results showed
significant (P≤0.05) decreased of the serum values of GSH, CAT and SOD as well,
significantly (P≤0.05) increased of MDA, peroxynitrate, creatinine and BUN of
group-II when compared to control; also the result of group-III showed nonsignificant
(P˃0.05) differences of GSH, SOD, MDA, creatinine, BUN and
peroxynitrate when compared to control. The histopathological and histochemical
results of kidney of group-II showed moderate thickness of basement membrane of
glomeruli with compressed capillaries as well infiltration of glyco-proteinaceous
materials in glomeruli and around renal tubules with some vacuolation of these
tubules and mesangial cells; while the results of group-III showed normal
architectures with very mild degree of vacuolation of few renal tubules in addition to
disappearance of glyco- proteinaceous materials in this group; in conclusion, ALA
had a dual protective effects on nephropathy by scavenging the oxidative stress free
radicals and enhanced insulin metabolism.

ISOLATION, IDENTIFICATION OF SOME FUGAL ISOLATES AND TESTING THEIR ABILITY FOR LIPOXYGENASE PRODUCTION

Amal K. G. AL-Asady; Alaa G.E. AL-Hashimi; Nora T.Y. AL-Temime

Basrah Journal of Veterinary Research, Volume 16, Issue 1, Pages 120-135
DOI: 10.33762/bvetr.2017.127442

Eighty three local isolates of fungi were isolated from different resources
(Peanuts , maize, rice, wheat, bread , domestic cheese of sheep, Milk local Cream,
Iranian cream, Roquefort cheese and soil). These isolates were purified and
identified, it include 14 isolates of Aspergillus flavus, 13 Aspergillus niger,8
Aspergillus terreus,3Aspergillus parasaticus,3Alternaria
spp.,15Penicilliumspp.,7Fusarium spp.,5 Trichoderma spp., 11 Rhizopus spp. and
7Mucor spp. The ability of isolates for producing aflatoxin were tested, the toxic
isolates(Aspergillus flavus,Aspergillus terreus ,and Aspergillus parasaticus) were
removed.Aspergillus niger which was isolated from maize was choosing as the best
lipoxygenase producer after Primary and secondary screening. The growth of the
selected isolate colonies had the largest proportion than the Colonies of Penicillium
sp. and Trichoderma sp..all so the same isolate had high enzymatic activity
801.4units/ml, while Penicillium sp. and Trichoderma sp. had (559.2 and 120)
units/ml respectively.

AN IMMUNOHISTOCHEMICAL LOCALIZATION OF ENDOCRINAL CELLS IN THE EPITHELIUM OF THE DUODENUM MUCOSA OF TURKEY ( Meleagaris gallpava )

Abdulrazzaq Baqer Kadhim

Basrah Journal of Veterinary Research, Volume 16, Issue 1, Pages 136-145
DOI: 10.33762/bvetr.2017.127443

in some parts of the intestinal tract, which play an important and primary role in the
secretion of certain hormones that regulate important organs of the body. The aim of
this study is to examine the site endocrine cells in the mucous layer of the five male
Turkey. The areas where endocrine cells in the mucous layer of the five male in the
Turkish examined by using immunohistochemistry technique material. Samples were
taken from five birds from the Turkish males ages ranging from 1-2 years. It was used
immunohistochemistry textile technology and (ChromograinnA), which is a special
detector Endocrine intestinal cells, four types of hormones. Immune chemical
examination in the mucous layer duodenum and the presence of hormones, glucose
insulin tropic polypeptide(GIP) results showed (GIP) and cholecystokinin (CCK)
glucagon-like peptide2 (GLP-2) in epithelial cells group along the internal axis of
the villi, chemical immunological results for many of the sections shown note
chromogranin A detector particularly for intestinal endocrine cells, the cells that
cholecystokinin(CCK) , glucose insulin tropic polypeptide contain hormones (GIP)
and glucagon peptide GLP-1 2)) containing this reagent indicating confirm the
location of these cells, a gastric endocrine cells . The presence of these hormones in
the mucous layer of the Duodenal decreases as we move away from the duodenal

HISTOLOGICAL STUDY OF THYROID GLAND IN CASE OF EXPERIMENTALLY INDUCED HYPOTHYROIDISM BY CARBIMAZOLE IN DOMESTIC FEMALE RABBITS (Lepus cuniculus domastica)

Abdul Jabar Rasmi; Fawzi S. Mehson; Shireen J. khaleel

Basrah Journal of Veterinary Research, Volume 16, Issue 1, Pages 146-156
DOI: 10.33762/bvetr.2017.127445

The present study aimed to investigate the histological ,histochemical changes of thyroid gland in case of induced hypothyroidism by carbimazole in domestic female rabbits .
Histological results revealed many histpathological changes compared with the control group there are hyperatrophy of follicular epithelium , hemorrhage ,edema and damage in the epithelium lining of the follicles.
Histochemical results revealed alterations in normal distribution of glycoprotein inside follicles of thyroid gland in hypothyrodic groups.